Amyloid diagnostics with single-molecule sensitivity
AmyloiDia Sweden AB Steering Board. From the left: Lars Terenius, Ann Tiiman, Vladana Vukojević, Jüri Jarvet, Rudolf Rigler, Astrid Gräslund and Sebastian Wärmländer. Missing: Johann Rigler.
Researchers from Karolinska Institute and Stockholm University non-disruptively monitor the turnover of intermediates during amyloid beta (Aβ) peptide aggregation in solution, simultaneously following the change in aggregate concentration and size over time:
Tiiman A, Jarvet J, Gräslund A, Vukojević V.
Heterogeneity and Turnover of Intermediates during Amyloid-β (Aβ) Peptide Aggregation Studied by Fluorescence Correlation Spectroscopy.
Biochemistry 2015 54(49): 7203-11 DOI: 10.1021/acs.biochem.5b00976).
Application in biological fluids was successfully developed and proof-of-concept measurements in blood serum, cerebrospinal fluid (CSF), urine and saliva were performed.
Priority Patent Application was filed on the discovery of a method for the diagnosis of amyloid-associated diseases (British Priority Application No 1805466.8).
International Patent Application under the Patent Cooperation Treaty (PCT) was filed on the discovery of a method for the diagnosis of amyloid-associated diseases (No. PCT/EP2019/058206).
Methodological proof-of-concept study published, showing in clinical blood serum samples that the concentration and size of amyloid aggregates can be successfully measured in blood serum using our methodology:
Tiiman A, Jelić V, Jarvet J, Järemo P, Bogdanović N, Rigler R, Terenius L,
Gräslund A, Vukojević V.
Amyloidogenic Nanoplaques in Blood Serum of Patients with Alzheimer's Disease Revealed by Time-Resolved Thioflavin T Fluorescence Intensity Fluctuation Analysis.
Journal of Alzheimer's disease: JAD 2019 68(2): 571-582 DOI: 10.3233/JAD-181144).
Instrumentation advancement by massive parallelization was published:
Krmpot AJ, Nikolić SN, Oasa S, Papadopoulos DK, Vitali M, Oura M, Mikuni S, Thyberg P,
Tisa S, Kinjo M, Nilsson L, Terenius L, Rigler R, Vukojević V.
Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells.
Analytical chemistry 2019 91(17): 11129-11137 DOI: 10.1021/acs.analchem.9b01813)
AmyloiDia Sweden AB founded (2019-08-27).
Method validation on clinical cerebrospinal fluid (CSF) samples and comparison with amyloid PET was published:
Aksnes M, Müller EG, Tiiman A, Edwin TH, Terenius L, Revheim ME, Vukojević V, Bogdanović N,
Amyloidogenic Nanoplaques in Cerebrospinal Fluid: Relationship to Amyloid Brain Uptake and Clinical Alzheimer's Disease in a Memory Clinic Cohort.
Journal of Alzheimer's Disease: JAD 2020 77(2): 831-842 DOI: 10.3233/JAD-200237).
Patent transferred to AmyloiDia.
First services to an early adopter.
First equity investment.
National patent filing phase entered.